The Braves star left Saturday's game and is awaiting an MRI to determine how much time he could miss.
Atlanta may need Austin Riley to help replace Acuna's production after two strong nights in Colorado, including a home run on Saturday.
The injury comes despite the Braves' hot start, powered by Matt Olson, Drake Baldwin, Michael Harris and Ozzie Albies, though some offensive output may be hard to sustain without Acuna.
With Acuña sidelined, will the Braves' risky 'no elite pitchers' strategy finally derail their dominant season?
Can Austin Riley's recent power surge fill the massive offensive void left by Ronald Acuña Jr.'s injury?
Is Ronald Acuña Jr.'s injury history turning his long-term contract into a high-stakes gamble for Atlanta?