The 24-1 long shot won Saturday at Churchill Downs in Louisville, beating 17 rivals after six scratches reduced the field to 18.
Renegade finished second, followed by Ocelli, Chief Wallabee and Danon Bourbon in the Run for the Roses.
The victory also made trainer Cherie DeVaux the first woman to win the race, while Golden Tempo later collected $3.1 million from the $5 million purse.
Beyond her barrier-breaking win, how will trainer Cherie DeVaux's methods impact the sport's debate on horse welfare?
Will a 25-1 longshot's victory force the racing industry to rethink how it evaluates its horses?
How will a historic win and new Preakness ownership reshape the Triple Crown's future?